Staining Reagents
Sigma Aldrich Fine Chemicals Biosciences BioTracker Green Copper Li
Copper is one of the trace metals required by living organisms and is an essential cofactor for enzyme activities of cytochrome c oxidase superoxide dismutase and tyrosinase. On the other hand abnormal homeostasis of copper leads to severe disorders such as Menkes syndrome and Wilsons disease or may be related to Alzheimers disease and cancers.BioTracker Green Copper Live Cell Dye is a fluorescent probe to detect Cu which is a dominant redox state of copper in an intracellular reducing environment. The dye is a colorless material with almost no fluorescence. Upon the reaction with Cu its fluorescence intensity at 510 nm increases more than 100 times to that before the reaction. However fluorescence increase is not observed in the presence of reactive oxygen species and other trace metals in cells including Cu2 Mn2 Co2 Ni2 fe fe2 and Zn2.Spectral PropertiesAbsorbance (max) 480nmEmission (max) 510nmResearch CategoryLive Cell Dye
Sigma Aldrich Fine Chemicals Biosciences BioTracker NucViewR 405 Bl
Caspases are a family of protease enzymes that play essential roles in programmed cell death termed apoptosis. Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9 and sequential activation of plays a central role in the execution-phase of cellular apoptosis.The BioTracker NucView 405 Blue Caspase-3 Dye provides a convenient live cell tool for profiling apoptotic cell populations based on caspase-3/7 activity using either fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (fLICA) assays the BioTracker NucView 405 Caspase-3 Dye can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. The substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate which is initially non-fluorescent penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells caspase-3/7 cl
Electron Microscopy Sciences Tissue Marking Dye Set
Fast drying dye is ideal for marking tissue biopsies and pathology specimens and can be used with frozen or fixed tissues. It is a specially formulated binding pigment that evenly coats the tissue without penetrating the tissue surface. The marking dyes do not interfere with diagnostic interpretation due to the fact colors are not similar to standard histopathology dyes. It can be used to permanently mark the margins of excised surgical specimens for exact orientation.
Fluotics PerkinElmer Janus Varispan compatible P1000, sterile
PerkinElmer Janus Varispan compatible P1000, sterile, Working Volume: 1000uL, Tip Color: Black, Hanging Rack, Varispan, Compare To: 6001604, 24 racks/case with 96 tips per rack
Sigma Aldrich Fine Chemicals Biosciences BioTracker 405 Blue Mitoch
Mitochondria are organelles found in large numbers in most cells in which the biochemical processes of respiration and energy production occurs. In metabolically active cells mitochondria produce a membrane potential by maintaining a proton gradient across the inner and outer membranes. Loss of mitochondrial membrane potential is a hallmark for apoptosis and poor cell health.BioTracker Mitochondria dyes are fluorogenic stains for staining mitochondria in live cells. The dyes are membrane permeable and become brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is lost when mitochondria become depolarized during cell death allowing monitoring of cell viability. The BioTracker 405 Blue Mitochondria Dye can be detected using the blue DAPI channel.Spectral PropertiesAbsorbance 398nmEmission 440nm
Electron Microscopy Sciences Acid Alcohol, 1% HCl/95% Alcohol 1 LT
Acidified alcohol solution is used for the differentiation of regressive stains. The solutions are freshly prepared upon receipt of order to ensure maximum shelf life.
American Research Products Inc BETA N ACETYLGLUCOSAMINIDASE K
5000265298 BETA N ACETYLGLUCOSAMINIDASE K